Peptide Reconstitution Calculator

There is no single correct amount, because the water only determines how concentrated the solution is, not how much peptide you get. Common choices are 1 mL, 2 mL, or 3 mL, and the practical goal is to land on a number that makes your prescribed dose an easy figure to read on the syringe rather than an awkward fraction of a unit. A 10 mg vial mixed with 2 mL gives 5,000 mcg per mL, so one unit on a U-100 syringe holds 50 mcg. The same vial mixed with 1 mL gives 100 mcg per unit. Use the dilution your provider specified, because your unit count was calculated from it.

The arithmetic works the same as any other vial. A 5 mg vial holds 5,000 mcg. Mixed with 2 mL of bacteriostatic water that gives 2,500 mcg per mL, so one unit on a U-100 syringe carries 25 mcg. Mixed with 1 mL instead it gives 5,000 mcg per mL and one unit carries 50 mcg. Aim the water down the inside wall of the vial rather than onto the powder, swirl gently, never shake, and confirm the solution goes completely clear before use. Our nightly protocol details are on the CJC-1295 and ipamorelin page, and the dose itself is provider-determined rather than something to select from a chart.

It depends completely on the concentration in your vial, which is why this question cannot be answered without knowing your dilution. On a U-100 insulin syringe, one unit is 0.01 mL of volume, not a fixed amount of peptide. If your vial is 5,000 mcg per mL, one unit holds 50 mcg and 250 mcg is 5 units. If it is 2,500 mcg per mL, one unit holds 25 mcg and the same 250 mcg dose becomes 10 units. Never carry a unit count over from a previous vial, because a new vial mixed at a different dilution changes the number even when your prescribed dose has not changed.

Bacteriostatic water contains a small amount of preservative, usually benzyl alcohol, which stops bacterial growth and allows the vial to be punctured repeatedly over a period of weeks. Sterile water has no preservative, so once the vial is entered there is nothing preventing contamination, which makes it suitable only for single use. For a multi-dose peptide vial that you will draw from repeatedly, bacteriostatic water is what belongs in it. The two are not interchangeable, and using sterile water for a vial you plan to use over several weeks is a genuine contamination risk rather than a technicality.

Once mixed, peptide solution has a limited shelf life measured in weeks rather than months, and the beyond-use date your provider gave you for your specific product is the number that governs rather than a figure from a forum. Keep it refrigerated on a shelf rather than in the door where the temperature swings, and away from light. Never freeze reconstituted solution, because freezing can damage the peptide even if it thaws looking clear. Write the mix date directly on the vial and stop using it when the window closes, even if liquid remains. Unmixed lyophilized powder follows entirely different storage rules.

No. Peptides are long chains of amino acids held in a specific shape, and that shape is what makes them work. Shaking creates shear forces and foaming that can break the chains or unfold them, leaving you with a vial that looks fine but has lost potency. Add the bacteriostatic water slowly down the inside wall of the vial rather than directly onto the powder, then either swirl gently or simply set it down and wait. Most peptides dissolve on their own within a couple of minutes. If a solution will not go clear after gentle swirling, that is a reason to stop and ask rather than a reason to shake harder.